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鍑濊啝鎴愬儚绯荤当(t菕ng)
闁辫畝锛�1264鏅�(sh铆)闁擄細2022-05-07 14:17:50

    鍑濊啝鎴愬儚鍗筹細灏�(du矛)DNA鎴朢NA鑶犺タ鐩�(sim)鍑濊啝鎴愬儚閫�(j矛n)琛屽垏鑶�銆佹媿鐓с�佽瀵�銆佸垎鏋� 锛岀殑瀵�(sh铆)椹�(y脿n)瀹ら(l猫i)鍎�鍣�锛� 鍑濊啝鎴愬儚绯荤当(t菕ng)鍙互鎳�(y墨ng)鐢ㄤ簬鍒嗗瓙 閲忚▓(j矛)绠楀瘑搴︽巸鎻忓瘑搴﹀畾閲� PCR瀹氶噺绛夌敓鐗╁伐绋嬪父瑕�(gu墨)鐮旂┒?锛熷偖w涓婁締(l谩i)瑾�(shu艒)鍑濊啝鎴愬儚鍙噳(y墨ng)鐢ㄤ簬锛氬嚌鑶犳垚鍍忕郴绲�(t菕ng)鍙互鐢ㄤ簬锛氳泲鐧借唱(zh矛)銆佹牳閰搞�佸鑲�銆佹皑鍩洪吀銆佸鑱氭皑鍩洪吀绛夊叾浠栫敓鐗╁垎瀛愮殑鍒嗛洟绱斿寲绲�(ji茅)鏋滀綔瀹氭�у垎鏋� 锛�1锛夊垎瀛愰噺瀹氶噺 灏�(du矛)浜庝竴鑸父鐢ㄧ殑DNA鑶犵墖鍒╃敤鍒嗗瓙閲忓畾閲忓姛鑳介�氶亷(gu貌)灏�(du矛)鑶犱笂DNA M

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    鍑濊啝鎴愬儚绯荤当(t菕ng)鍙互鎳�(y墨ng)鐢ㄤ簬鍒嗗瓙

    閲忚▓(j矛)绠�,瀵嗗害鎺冩弿,瀵嗗害瀹氶噺,

    PCR瀹氶噺绛夌敓鐗╁伐绋嬪父瑕�(gu墨)鐮旂┒銆�


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    绺介珨涓婁締(l谩i)瑾�(shu艒)鍑濊啝鎴愬儚鍙噳(y墨ng)鐢ㄤ簬锛氬嚌鑶犳垚鍍忕郴绲�(t菕ng)鍙互鐢ㄤ簬锛氳泲鐧借唱(zh矛)銆佹牳閰�銆佸鑲�銆佹皑鍩洪吀銆佸鑱氭皑鍩洪吀绛夊叾浠栫敓鐗╁垎瀛愮殑鍒嗛洟绱斿寲绲�(ji茅)鏋滀綔瀹氭�у垎鏋�

    锛�1锛夊垎瀛愰噺瀹氶噺

    灏�(du矛)浜庝竴鑸父鐢ㄧ殑DNA鑶犵墖,鍒╃敤鍒嗗瓙閲忓畾閲忓姛鑳�,閫氶亷(gu貌)灏�(du矛)鑶犱笂DNA Marker姊濆付鐨勫凡鐭ュ垎瀛愰噺娉ㄩ噵,鑷嫊(d貌ng)鐢熸垚鎿悎鏇茬窔,骞朵互瀹冭　閲忓緱鍒版湭鐭ユ甯剁殑鍒嗗瓙閲�銆傞�氶亷(gu貌)閫欑ó鏂规硶鎵�寰楀埌鐨勭祼(ji茅)鏋滆純鑲夌溂瑙�瀵熶及瑷�(j矛)瑕佹簴(zh菙n)纰哄緢澶�銆�

    锛�2锛夊瘑搴﹀畾閲�

    涓�鑸父鐢ㄧ殑娓�(c猫)瀹欴NA(鑴哀鏍哥硸鏍搁吀)鍜孯NA(鏍哥硸鏍搁吀)婵冨害鐨勬柟娉曟槸绱鍚告敹娉�,浣嗗畠鍙兘娓�(c猫)瀹氭ǎ鍝佷腑鐨勭附鏍歌嫹閰告績搴�,鑰屼笉鑳藉崁(q奴)鍒嗗悇鍊�(g猫)闀�(zh菐ng)搴︾墖娈电殑婵冨害銆傚埄鐢ㄥ嚌鑶犳垚鍍忕郴绲�(t菕ng)鍜岃粺浠�,鍏堝皣DNA鑶犵墖涓婃煇涓�宸茬煡鍏禗NA鍚噺鐨勬(bi膩o)婧�(zh菙n)姊濆付閫�(j矛n)琛屽瘑搴︽(bi膩o)瀹氫互鍚�,鍙互鏂逛究鐨勫柈鎿婂叾浠栨湭鐭ユ甯�锛屾牴鎿�(j霉)鑸囧凡鐭ユ甯剁殑瀵嗗害鍋氭瘮杓�锛屽彲浠ュ緱鍒版湭鐭NA鐨勫惈閲�銆傛鏂规硶涔熼仼鐢ㄤ簬灏�(du矛)PA GE铔嬬櫧鑶犳甯剁殑婵冨害娓�(c猫)瀹氥��

    锛�3锛夊瘑搴︽巸鎻�

    鍦ㄥ垎瀛愮敓鐗╁(xu茅)鍜岀敓鐗╁伐绋嬬爺绌朵腑,甯哥敤鍒扮殑鏄皪(du矛)铔嬬櫧琛ㄩ仈(d谩)鐢�(ch菐n)鐗╁崰鏁村��(g猫)鑿岄珨铔嬬櫧鐨勭櫨鍒嗗惈閲忕殑瑷�(j矛)绠�銆傚偝绲�(t菕ng)鐨勬柟娉曟槸鍒╃敤灏�(zhu膩n)鐢ㄧ殑瀵嗗害鎺冩弿,浣嗗埄鐢ㄧ敓鐗╁垎鏋愯粺浠剁祼(ji茅)鍚堢従(xi脿n)鍦ㄥ(sh铆)椹�(y脿n)瀹ゅ父瑕�(gu墨)閰嶅倷鐨勬巸鎻忓剙鎴栬�呯洿鎺ョ敤鐧藉厜鐓у皠鐨勫嚌鑶犳垚鍍忓氨鑳藉畬鎴愭闋�(xi脿ng)宸ヤ綔銆�

    锛�4锛塒CR瀹氶噺

    PCR瀹氶噺涓昏鏄寚锛屽鏋淧CR瀵�(sh铆)椹�(y脿n)鎿�(ku貌)澧炲嚭渚�(l谩i)鐨勬甯朵笉鏄竴姊濓紝閭ｄ箞鍙互鍒╃敤杌熶欢瑷�(j矛)绠楀嚭鍚勫��(g猫)姊濆付鍗犵附楂旀甯剁殑鐩稿皪(du矛)鐧惧垎?j菒n)?sh霉)銆傚氨姝ゅ姛鑳借�岃█锛岃垏瀵嗗害鎺冩弿椤�(l猫i)浼硷紝浣嗗(sh铆)闅涘湪鍘熺悊涓婂苟涓嶇浉鍚�銆侾CR瀹氶噺鏄皪(du矛)閬稿畾鐨勫咕姊濆付閫�(j矛n)琛岀浉灏�(du矛)瀵嗗害瀹氶噺骞惰▓(j矛)绠楀叾鍗犵附鍜岀殑鐧惧垎?j菒n)?sh霉)锛屽瘑搴︽巸鎻忔檪(sh铆)骞跺皪(du矛)閬告搰鍗�(q奴)鍩熺敓鎴愮副鍚戞巸鎻忔洸绶氬湒骞剁⿳鍒嗐��


鍑濊啝鎴愬儚绋(l猫i)

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    锛�3锛夊鑹茬啋鍏夋垚鍍忓垎鏋愮郴绲�(t菕ng)锛氭垚鍍忚寖鍦嶆兜钃婾V,EB,鍖栧(xu茅)鐧�(f膩)鍏�銆佸鑹茬啋鍏夌啋鍏夈�佹湁鑹插強鍙(ji脿n)妯ｅ搧鎴愬儚锛�

    锛�4锛夊鍔熻兘娲婚珨鎴愬儚鍒嗘瀽绯荤当(t菕ng)锛歎V,EB,鍖栧(xu茅)鐧�(f膩)鍏�銆佸鑹茬啋鍏夌啋鍏�銆佹湁鑹插強鍙(ji脿n)妯ｅ搧鎴愬儚鍜岄洟楂旂祫绻斿拰灏忓瀷鍕�(d貌ng)鐗�锛屽強澶у瀷鍨嬪嫊(d貌ng)鐗�銆�


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